Find our most recent list of publications here.
(bold face = Struct Plant Bio Lab Members, †co-first author, *corresponding author)
Preprints (non peer-reviewed manuscripts)
- Okuda S, Hothorn LA, Hothorn M* (2020) Crystal structures of Arabidopsis and Physcomitrella CR4 reveal the molecular architecture of CRINKLY4 receptor kinases. bioRxiv doi: https://doi.org/10.1101/2020.08.10.245050
Research publications (peer-reviewed)
- Raia P, Lee K, Bartsch SM, Rico-Resendiz F, Portugal-Calisto D, Vadas O, Panse VG, Fiedler D, Hothorn, M* (2025) A small signaling domain PPIP5K phosphatase activity in phosphate homeostasis. Nat Commun, doi: 10.1038/s41467-025-56937-0 | bioRxiv
- Yang X, Stentenbach M, Hughes LA, Siira SJ, Lau K, Hothorn M, Martinou JC, Rackham O, Filipovska A (2025) The Vsr-like protein FASTKD4 regulates the stability and polyadenylation of the MT-ND3 mRNA. Nucleic Acids Res. doi: https://doi.org/10.1093/nar/gkae1261
- Laurent F, Bartsch SM, Shukla A, Rico-Resendiz F, Couto D, Fuchs C, Nicolet J, Loubery S, Jessen HJ, Fiedler D, Hothorn M* (2024) Inositol pyrophosphate catabolism by three families of phosphatases regulates plant growth and development. Plos Genetics doi: https://doi.org/10.1371/journal.pgen.1011468 | bioRxiv
- Yu J, Kumar A, Zhang Z, Martin C, Van Holsbeeck K, Raia P, Koehl A, Laeremans T, Stevaert J, Manglink A, Ballet S, Boland A, Stoeber M (2024) Structural basis of mu-opiod receptor targeting by a nanobody antagonist. Nat Commun 15:8687 doi: https://doi.org/10.1038/s41467-024-52947-6
- Obergfell E, Hohmann U, Moretti A, Chen H, Hothorn M* (2024) Mechanistic insights into the function of 14-3-3 proteins as negative regulators of brassinosteroid signaling in Arabidopsis. Plant & Cell Physiol, pcae056, https://doi.org/10.1093/pcp/pcae056 | bioRxiv doi: https://doi.org/10.1101/2023.10.13.562204
- Guan Z, Chen J, Liu R, Chen Y, Xing Q, Du Z, Cheng M, Hu J, Zhang W, Mei W, Wan B, Wang Q, Zhang J, Cheng P, Cai H, Cao J, Zhang D, Yan J, Yin P, Hothorn M, Liu Z (2023) The cytoplasmic synthesis and coupled membrane translocation of eukaryotic polyphophate by signal-activated VTC complex. Nat Comms doi: 10.1038/s41467-023-36466-4
- Claus LAN, Liu D, Hohmann U, Vukasinovic N, Pleskot R, Liu J, Schiffner A, Jaillais Y, Wu G, Wolf S, Van Damme D, Hothorn M, Russinova E (2023) Phosphorylation and ubiquitination independent endocytosis of BRI1. Plant Physiol, doi: 10.1093/plphys/kiad005 | bioRxiv doi: 10.1101/2022.07.11.499622v1
- Pesquera M, Martinez J, Maillot B, Wang K, Hofmann M, Raia P, Loubery S, Steensma P, Hothorn M*, Fitzpatrick TB* (2022) Structural and functional insight into the plant unique multimodular triphosphosphate tunnel metalloenzymes of Arabidopsis thaliana. J Biol Chem doi: 10.1016/j.jbc.2022.102438 bioRxiv doi: https://doi.org/10.1101/2022.03.18.484887
- Guan Z, Zhang Q, Zhang Z, Zuo J, Chen J, Liu R, Savarin J, Broger L, Cheng P, Wang Q, Pei K, Zhang D, Zou T, Yan J, Yin P, Hothorn M, Liu Z (2022) Mechanistic insights into the regulation of plant phosphate homeostasis by the rice SPX2 – PHR2 complex. Nature Comms doi: 10.1038/s41467-022-29275-8
- Couto DUsing biotinylated myo-inositol hexakisphosphate to investigate inositol pyrophosphate-protein interaction with surface-based biosensors. Biochemisty 60(37):2739-2748. doi:10.1021/acs.biochem.1c00497 , Richter A , Walter H, Furkert D, Hothorn M*, Fiedler D* (2021)
- Podolec R†, Lau K†, Wagnon TB, Hothorn M*, Ulm R* (2021) A constitutively monomeric UVR8 photreceptor allele confers enhanced UV-B photomorphogenesis. PNAS doi: https://doi.org/10.1073/pnas.2017284118 | bioRxiv doi: https://doi.org/10.1101/2020.08.02.233007
- Ried M†, Wild R†, Zhu J, Pipercevic J, Sturm K, Broger L, Harmel RK, Abriata LA, Hothorn LA, Fiedler D, Hiller S, Hothorn M* (2021) Inositol pyrophosphates promote the interaction of SPX domains with the coiled-coil motif of PHR transcription factors to regulate plant phosphate homeostasis. Nature Comms 12(1):384 doi: https://doi.org/10.1038/s41467-020-20681-4 | bioRxiv
- BAM1/2 receptor kinase signaling drives CLE peptide-mediated formative cell divisions in Arabidopsis roots. PNAS 17(51):32750-32756 doi: 10.1073/pnas.2018565117
- 40(1):e104273
- Hohmann U, Ramakrishna P, Wang K, Lorenzo-Orts L, Nicolet J, Henschen A, Barberon M, Bayer M*, Hothorn M* (2020) Constitutive activation of leucine-rich repeat receptor kinase signaling pathways by BAK1-interacting receptor-like kinase 3 chimera Plant Cell 32(10):3311-3323 doi: 10.1105/tpc.20.00138| bioRxiv doi: https://doi.org/10.1101/2020.02.18.954479
- Doll NM, Royek S†, Fujita S†, Okuda S†, Stintzi A, Widiez T, Hothorn M, Schaller A, Geldner N, Ingram G (2020) A two-way molecular dialogue between embryo and endosperm required for seed development. Science 367:431-435 doi: https://doi.org/ 10.1126/science.aaz4131 | bioRxiv
- Okuda S†, Fujita S†, Moretti A, Hohmann U,Molecular mechanism for the recognition of sequence-divergent CIF peptides by the plant receptor kinases GSO1/SGN3 and GSO2. PNAS 17(5):2693-2703 doi: https://doi.org/10.1073/pnas.1911553117 | bioRxiv doi: https://doi.org/10.1101/692228v2 Hothorn M* (2020)
- Zhu J, Loubery S, Broger L, Lorenzo-Orts L, Utz-Pugin A, Chang Y-T, Hothorn M* (2020) A genetically validated approach to detect inorganic polyphosphates in plants. Plant J 102(3):507-516 doi: https://doi.org/10.1111/tpj.14642.| bioRxiv doi: https://doi.org/10.1101/630129v1
- Zhu J, Lau K, Puschmann R, Harmel RK, Zhang Y, Pries V, Gaugler P, Broger L, Dutta AK, Jessen HJ, Schaaf G, Fernie AR, Hothorn LA, Fiedler D, Hothorn M* (2019)
Two bifunctional inositol pyrophosphate kinases/phosphatases control plant phosphate homeostasis. eLife pii: e43582. | bioRxiv - Lau K†, Podolec R†, Chappuis R, Ulm R*, Hothorn M* (2019) Plant photoreceptors and their signaling components compete for binding to the ubiquitin ligase COP1 using their VP-peptide motifs. EMBO Journal, e102140. doi: 10.15252/embj.2019102140 | bioRxiv doi: https://doi.org/10.1101/568618
- Lorenzo-Orts L, Hohmann U, Zhu J, Hothorn M* (2019) Molecular characterization of CHAD domains as inorganic polyphosphate binding modules.
Life Sci Alliance. 2(3). pii: e201900385 | bioRxiv doi: https://doi.org/10.1101/567040 - Hohmann U, Hothorn M* (2019) Crystal structure of the LRR ectodomain of the plant immune receptor kinase SOBIR1.
Acta Crystallogr D Biol Crystallogr. 75(Pt 5):488-497. https://doi.org/10.1107/S2059798319005291 | bioRxiv https://doi.org/10.1101/581231 - Robinson GR, Kaufmann M, Roux C, Martinez J, Hothorn M, Thore S, Fitzpatrick TB (2019) Crystal structure of the pseudoenzyme PDX1.2 in complex with its cognate enzyme PDX1.3: a total eclipse
Acta Crystallogr D Biol Crystallogr.75(Pt 4):400-415.
- Lorenzo-Orts L*, Witthoeft J, Deforges J, Martinez J, Loubery S, Placzek A, Poirier Y, Hothorn LA, Jaillais Y, Hothorn M* (2019) Concerted expression of a cell-cycle regulator and a metabolic enzyme from a bicistronic transcript in plants.
Nature Plants,5:184-193 (free read access) - Vaattovaara A, Brandt B, Rajaraman S, Safronov O, Veidenberg A, Lukova M, Kangasjärvi J, Löytynoja A, Hothorn M, Salojärvi S, Wrzaczek M (2019)
Mechanistic insights into the evolution of DUF26-containing proteins in land plants.
Communications Biology 2, article no. 59 | bioRxiv doi: https://doi.org/10.1101/493502 - Kozlowski M, Corujo D, Hothorn M, Guberovic I, Mandemaker IK, Blessing C, Sporn J, Gutierrez‐Triana A, Smith R, Portmann T, Treier M, Scheffzek K, Huet S, Timinszky G, Buschbeck M, Ladurner AG (2018)
MacroH2A histone variants limit chromatin plasticity through two distinct mechanisms
EMBO Reports 10.15252/embr.201744445 (free read access) - Anne P, Amiguet-Vercher A, Brandt B, Kalmbach L, Geldner N, Hothorn M, Hardtke CS (2018)
CLERK is a novel receptor kinase required for sensing of root-active CLE peptides in Arabidopsis.
Development; 145(10). pii: dev162354. doi: 10.1242/dev.162354. open-access full-text - Hohmann U, Nicolet J, Moretti A, Hothorn LA, Hothorn M* (2018)
The SERK3 elongated allele defines a role for BIR ectodomains in brassinosteroid signalling.
Nature Plants 4:345-351 doi: 10.1038/s41477-018-0150-9 | bioRxiv doi: https://doi.org/10.1101/257543 - Hohmann U, Santiago J, Nicolet J, Olsson V, Spiga FM, Hothorn LA, Butenko MA, Hothorn M* (2018)
Mechanistic basis for the activation of plant membrane receptor kinases by SERK-family coreceptors.
PNAS 115(13):3488-3493, free full-text
- Hazak O†, Brandt B†, Cattaneo P, Santiago J, Rodriguez-Villalon A, Hothorn M*, Hardtke CS* (2017)
Perception of root-active CLE peptides requires CORYNE function in the phloem vasculature.
EMBO Reports, - Wild R, Hothorn M* (2017)
The macro domain as fusion tag for carrier-driven crystallization.
Protein Sci 26(2):365-374, open access full-text - Wild R†, Gerasimaite R†, Jung JY†, Truffault V, Pavlovic I, Schmidt A, Saiardi A, Jessen HJ, Poirier Y*, Hothorn M*, Mayer A* (2016)
Control of eukaryotic phosphate homeostasis by inositol polyphosphate sensor domains.
Science 352(6288):986-90 author manuscript - Santiago J*, Brandt B, Wildhagen M, Hohmann U, Hothorn LA, Butenko MA, Hothorn M* (2016)
Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission.
eLife doi: 10.7554/eLife.15075 open access full-text - Binkert M, Crocco CD, Ekundayo B, Lau K, Raffelberg S, Tilbrook K, Yin R, Chappuis R, Schalch T, Ulm R (2016)
Revisiting chromatin binding of the Arabidopsis UV-B photoreceptor UVR8.
BMC Plant Biol doi: 10.1186/s12870-016-0732-5 - Martinez J, Truffault V, Hothorn M* (2015)
Structural Determinants for Substrate Binding and Catalysis in Triphosphate Tunnel Metalloenzymes.
J Biol Chem. 290:23348-60 open access full-text - Bojar D, Martinez J, Santiago J, Rybin V, Bayliss R, Hothorn M* (2014)
Crystal structures of the phosphorylated BRI1 kinase domain and implications for brassinosteroid signal initiation.
Plant J. 78:31-43 open access full-text - Santiago J, Henzler C, Hothorn M* (2013)
Molecular Mechanism for Plant Steroid Receptor Activation by Somatic Embryogenesis Co-Receptor Kinases.
Science 341:889-92 free full-text (with registration) author manuscript - Christie JM, Arvai AS, Baxter KJ, Heilmann M, Pratt AJ, O’Hara A, Kelly SM, Hothorn M, Smith BO, Hitomi K, Jenkins GI, Getzoff ED (2012)
Plant UVR8 Photoreceptor Senses UV-B by Tryptophan-Mediated Disruption of Cross-Dimer Salt Bridges.
Science 335:1492-1496 - Feldman-Salit A, Wirtz M, Lenherr ED, Throm C, Hothorn M, Scheffzek K, Hell R, Wade RC (2012)
Allosterically Gated Enzyme Dynamics in the Cysteine Synthase Complex Regulate Cysteine Biosynthesis in Arabidopsis thaliana.
Structure 20(2):292-302 - Cuyvers S, Dornez E, Abou Hachem M, Svensson B, Hothorn M, Chory J, Delcour JA, Courtin CM (2012)
Isothermal titration calorimetry and surface plasmon resonance allow quantifying substrate binding to different binding sites of Bacillus subtilis xylanase.
Anal Biochem. 420(1):90-92 - Hothorn M, Dabi T, Chory J (2011)
Structural basis for cytokinin recognition by Arabidopsis thaliana histidine kinase 4.
Nat Chem Biol. 7(11):766-768 - Hothorn M, Belkhadir Y, Dreux M, Dabi T, Noel JP, Wilson IA, Chory J (2011)
Structural basis of steroid hormone perception by the receptor kinase BRI1.
Nature 474(7352):467-71 - Jaillais Y†, Hothorn M†, Belkhadir Y, Dabi T, Nimchuk ZL, Meyerowitz EM, Chory J (2011)
Tyrosine phosphorylation controls brassinosteroid receptor activation by triggering membrane release of its kinase inhibitor.
Genes Dev. 25(3):232-237 - Hothorn M†, Van den Ende W†, Lammens W, Rybin V, Scheffzek K (2010)
Structural insights into the pH-controlled targeting of plant cell-wall invertase by a specific inhibitor protein.
Proc Natl Acad Sci U S A. 107(40):17427-17432 - Reinelt S, Koch B, Hothorn M, Hengstenberg W, Welti S, Scheffzek K. (2009)
Structure of the Enterococcus faecalis EIIA(gnt) PTS component.
Biochem Biophys Res Commun. 388(4):626-629 - Timinszky G†, Till S†, Hassa PO†, Hothorn M†, Kustatscher G, Nijmeijer B, Colombelli J, Altmeyer M, Stelzer EH, Scheffzek K, Hottiger MO, Ladurner AG (2009)
A macrodomain-containing histone rearranges chromatin upon sensing PARP1 activation.
Nat Struct Mol Biol. 16(9):923-929 - Hothorn M, Neumann H, Lenherr ED, Wehner M, Rybin V, Hassa PO, Uttenweiler A, Reinhardt M, Schmidt A, Seiler J, Ladurner AG, Herrmann C, Scheffzek K, Mayer A (2009)
Catalytic core of a membrane-associated eukaryotic polyphosphate polymerase.
Science 324(5926):513-516 - Corsini L, Hothorn M, Stier G, Rybin V, Scheffzek K, Gibson TJ, Sattler M (2009)
Dimerization and protein binding specificity of the U2AF homology motif of the splicing factor Puf60.
J Biol Chem. 2;284(1):630-639 - Corsini L, Hothorn M, Scheffzek K, Sattler M, Stier G (2008)
Thioredoxin as a fusion tag for carrier-driven crystallization.
Protein Sci. 17(12):2070-2079 - Stuwe T†, Hothorn M†, Lejeune E, Rybin V, Bortfeld M, Scheffzek K, Ladurner AG (2008)
The FACT Spt16 “peptidase” domain is a histone H3-H4 binding module
Proc Natl Acad Sci U S A. 105(26):8884-8889 - Gromes R, Hothorn M, Lenherr ED, Rybin V, Scheffzek K, Rausch T (2008)
The redox switch of gamma-glutamylcysteine ligase via a reversible monomer-dimer transition is a mechanism unique to plants.
Plant J. 54(6):1063-1071. - Pena V, Hothorn M, Eberth A, Kaschau N, Parret A, Gremer L, Bonneau F, Ahmadian MR, Scheffzek K (2008)
The C2 domain of SynGAP is essential for stimulation of the Rap GTPase reaction.
EMBO Rep. 9(4):350-355 - Till S, Lejeune E, Thermann R, Bortfeld M, Hothorn M, Enderle D, Heinrich C, Hentze MW, Ladurner AG (2007)
A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain.
Nat Struct Mol Biol. 14(10):897-903 - Corsini L, Bonnal S, Basquin J, Hothorn M, Scheffzek K, Valcárcel J, Sattler M (2007)
U2AF-homology motif interactions are required for alternative splicing regulation by SPF45.
Nat Struct Mol Biol. 14(7):620-629 - Simader H, Hothorn M, Suck D (2006)
Structures of the interacting domains from yeast glutamyl-tRNA synthetase and tRNA-aminoacylation and nuclear-export cofactor Arc1p reveal a novel function for an old fold.
Acta Crystallogr D Biol Crystallogr. 62(Pt 12):1510-1519 - Simader H, Hothorn M, Köhler C, Basquin J, Simos G, Suck D (2006)
Structural basis of yeast aminoacyl-tRNA synthetase complex formation revealed by crystal structures of two binary sub-complexes.
Nucleic Acids Res. 34(14):3968-3979 - Hothorn M, Wachter A, Gromes R, Stuwe T, Rausch T, Scheffzek K (2006)
Structural basis for the redox control of plant glutamate cysteine ligase.
J Biol Chem. 281(37):27557-27565 - Hothorn M, Scheffzek K (2006)
Multiple crystal forms of the cell-wall invertase inhibitor from tobacco support high conformational rigidity over a broad pH range.
Acta Crystallogr D Biol Crystallogr. 62(Pt 6):665-670 - Kustatscher G†, Hothorn M†, Pugieux C, Scheffzek K, Ladurner AG (2005)
Splicing regulates NAD metabolite binding to histone macroH2A.
Nat Struct Mol Biol. 12(7):624-625 - Hothorn M, Wolf S, Aloy P, Greiner S, Scheffzek K (2004)
Structural insights into the target specificity of plant invertase and pectin methylesterase inhibitory proteins.
Plant Cell 16(12):3437-3447 - Hothorn M, D’Angelo I, Márquez JA, Greiner S, Scheffzek K (2004)
The invertase inhibitor Nt-CIF from tobacco: a highly thermostable four-helix bundle with an unusual N-terminal extension.
J Mol Biol. 335(4):987-895 - Hothorn M, Bonneau F, Stier G, Greiner S, Scheffzek K (2003)
Bacterial expression, purification and preliminary X-ray crystallographic characterization of the invertase inhibitor Nt-CIF from tobacco.
Acta Crystallogr D Biol Crystallogr. 59(Pt 12):2279-2282
Reviews (peer-reviewed)
- Lorenzo-Orts L, Couto D, Hothorn M (2019)
Identity and functions of inorganic and inositol polyphosphates in plants. New Phytologist, doi: 10.1111/nph.16129.
- Jung JY, Ried MK, Hothorn M*, Poirier Y* (2017)
Control of plant phosphate homeostasis by inositol pyrophosphates and the SPX domain.
Current Opinion in Biotechnology 49:156-162, free full-text
- Hohmann U, Lau K, Hothorn M (2017)
The Structural Basis of Ligand Perception and Signal Activation by Receptor Kinases.
Ann Rev Plant Bio 18(8):1367-1381, open access full-text - Brandt B, Hothorn M (2016)
SERK co-receptor kinases.
Curr Biol. 26(6):R225-6 - Marshall A, Aalen RB, Audenaert D, Beeckman T, Broadley MR, Butenko MA, Caño-Delgado AI, de Vries S, Dresselhaus T, Felix G, Graham NS, Foulkes J, Granier C, Greb T, Grossniklaus U, Hammond JP, Heidstra R, Hodgman C, Hothorn M, Inzé D, Ostergaard L, Russinova E, Simon R, Skirycz A, Stahl Y, Zipfel C, De Smet I (2012)
Tackling Drought Stress: RECEPTOR-LIKE KINASES Present New Approaches.
Plant Cell 24:2262-2278
Book chapter
- Hohmann U, Hothorn M (2018)
Brassinosteroid sensing and signaling in plants.
in Plant Structural Biology: Hormonal Regulations by Jan Hejatko, Toshio Hakoshima (Eds.), Springer
PhD theses
- Laurent F (2024) Understanding the catabolism of inositol pyrophosphates in plants. PhD Thesis University of Geneva
- Lorenzo-Orts L (2019) Biochemical and physiological studies on polyphosphate metabolism in plants. PhD Thesis University of Geneva
- Hohmann U (2018) Mechanistic insight into the activation and negative regulation of plant leucine-rich repeat receptor kinases. PhD Thesis University of Geneva
- Wild R (2016) Structural and functional characterization of eukaryotic SPX domains as inositol polyphosphate sensors. PhD Thesis University of Geneva
- Hothorn M (2006) Crystallographic studies on protein regulatory mechanisms PhD thesis European Molecular Biology Laboratory and Ruperto Carola University of Heidelberg, Germany